at#:2112 Anti-DNA-RNA Hybrid [S9.6] Antibody
|
Synonyms: |
DNA-RNA hybrid; RNA/DNA hybrid |
|
Attribute: |
MERGEFIELD Antibody_type Mouse Monoclonal Antibody |
|
Isotype: |
Mouse IgG2a |
|
Purity: |
Protein A/G Purification |
|
Application: |
MERGEFIELD Tested_applications ELISA, IP, IF, CHIP, Dot Blot |
|
Immunogen： |
ΦX174 bacteriophage-derived synthetic DNA/RNA |
|
Buffer： |
PBS with 0.1% sodium azide 50% glycerol, pH 7.2 |
|
Storage: |
Store at -20℃. Do not aliquot |
|
Recommended Dilution: |
WB: 1:1000-5000 IP: 1:50-500 ChIP: 1:50-500 IF: 1:100-500 |
|
Background： The DNA-RNA hybrids are a natural occurrence within eukaryotic cells their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus toomosomal breakage. The S9.6 monoclonal antibody recognizes DNA-RNA hybrids (also known as R-loops) does not bind to single double stranded DNA. The antibody has high affinity fDNA-RNA hybrids but also binds RNA-RNA hybrids that are AU-rich. The specificity of the antibody appears to be determined by a combination of sequence structural dependency since R-loop sequence affects binding affinity. |
|
检测结果
Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using DNA-RNA hybrid MERGEFIELD Antibody_type Mouse Monoclonal Antibody [S9.6] at dilution of 1:500
Alexa Flu647- conjugated AffiniPure Goat Anti-Mouse IgG(H+L)
Chromatin immunoprecipitation analysis of HeLa cells genomic DNA(gDNA) using DNA-RNA hybrid MERGEFIELD Antibody_type Mouse Monoclonal
Antibody [S9.6] at dilution of 1:200
